Identification of cell lines permissive for human coronavirus NL63

Publikation

Identification of cell lines permissive for human coronavirus NL63

Wissenschaftlicher Artikel/Review - 07.09.2006

Schildgen Oliver, Jebbink Maarten F, de Vries Michel, Pyrc Krzysztov, Dijkman Ronald, Simon Arne, Müller Andreas, Kupfer Bernd, van der Hoek Lia

Zitation

Schildgen O, Jebbink M, de Vries M, Pyrc K, Dijkman R, Simon A, Müller A, Kupfer B, van der Hoek L. Identification of cell lines permissive for human coronavirus NL63. J Virol Methods 2006; 138:207-10.

Art

Wissenschaftlicher Artikel/Review (Englisch)

Zeitschrift

J Virol Methods 2006; 138

Veröffentlichungsdatum

07.09.2006

ISSN (Druck)

0166-0934

Seiten

207-10

Kurzbeschreibung/Zielsetzung

Six cell lines routinely used in laboratories were tested for permissiveness to the infection with the newly identified human coronavirus NL63. Two monkey epithelial cell lines, LLC-MK2 and Vero-B4, showed a cytopathic effect (CPE) and clear viral replication, whereas no CPE or replication was observed in human lung fibroblasts MRC-5s. In Rhabdomyosarcoma cells, Madin-Darby-Canine-kidney cells and in an undefined monkey kidney cell line some replication was observed but massive exponential rise in virus yield lacked The results will lead to an improved routine diagnostic algorithm for the detection of the human coronavirus NL63.