Identification of cell lines permissive for human coronavirus NL63

Publication

Identification of cell lines permissive for human coronavirus NL63

Journal Paper/Review - Sep 7, 2006

Schildgen Oliver, Jebbink Maarten F, de Vries Michel, Pyrc Krzysztov, Dijkman Ronald, Simon Arne, Müller Andreas, Kupfer Bernd, van der Hoek Lia

Citation

Schildgen O, Jebbink M, de Vries M, Pyrc K, Dijkman R, Simon A, Müller A, Kupfer B, van der Hoek L. Identification of cell lines permissive for human coronavirus NL63. J Virol Methods 2006; 138:207-10.

Type

Journal Paper/Review (English)

Journal

J Virol Methods 2006; 138

Publication Date

Sep 7, 2006

Issn Print

0166-0934

Pages

207-10

Brief description/objective

Six cell lines routinely used in laboratories were tested for permissiveness to the infection with the newly identified human coronavirus NL63. Two monkey epithelial cell lines, LLC-MK2 and Vero-B4, showed a cytopathic effect (CPE) and clear viral replication, whereas no CPE or replication was observed in human lung fibroblasts MRC-5s. In Rhabdomyosarcoma cells, Madin-Darby-Canine-kidney cells and in an undefined monkey kidney cell line some replication was observed but massive exponential rise in virus yield lacked The results will lead to an improved routine diagnostic algorithm for the detection of the human coronavirus NL63.