Reverse genetics of coronaviruses using vaccinia virus vectors

Publikation

Reverse genetics of coronaviruses using vaccinia virus vectors

Buchkapitel - 01.01.2005

Thiel Volker, Siddell S G

Bereiche

Institut für Immunbiologie

PubMed

15609513

Zitation

Thiel V, Siddell S. Reverse genetics of coronaviruses using vaccinia virus vectors. In: Coronavirus Replication and Reverse Genetics. Heidelberg: Springer, 2004. ISBN 978-3-540-21494-6. S. 199-227.

Art

Buchkapitel (Englisch)

Buchtitel

Coronavirus Replication and Reverse Genetics

Veröffentlichungsdatum

01.01.2005

Titel der Reihe

Current Topics in Microbiology and Immunology (287)

ISBN Nummer

978-3-540-21494-6

Seiten

199-227

Verlag

Springer (Heidelberg)

Kurzbeschreibung/Zielsetzung

In this article, we describe the reverse genetic system that is based on the use of vaccinia virus cloning vectors. This system represents a generic approach to coronavirus reverse genetics and was first described for the generation of recombinant human coronavirus 229E representing a group I coronavirus. Subsequently, the same approach has been used to generate recombinant avian infectious bronchitis coronavirus and, recently, recombinant mouse hepatitis virus, representing group III and group II coronaviruses, respectively. We describe how vaccinia virus-mediated homologous recombination can be used to introduce specific mutations into the coronavirus genomic cDNA during its propagation in vaccinia virus and how recombinant coronaviruses can be isolated. Finally, we describe how the coronavirus reverse genetic system has now been extended to the generation of coronavirus replicon RNAs.