Reverse genetics of coronaviruses using vaccinia virus vectors

Publication

Reverse genetics of coronaviruses using vaccinia virus vectors

Book Chapter - Jan 1, 2005

Thiel Volker, Siddell S G

Units

Institute of Immunobiology

PubMed

15609513

Citation

Thiel V, Siddell S. Reverse genetics of coronaviruses using vaccinia virus vectors. In: Coronavirus Replication and Reverse Genetics. Heidelberg: Springer, 2005. ISBN 978-3-540-21494-6. pp. 199-227.

Type

Book Chapter (English)

Book Title

Coronavirus Replication and Reverse Genetics

Publication Date

Jan 1, 2005

Series Title

Current Topics in Microbiology and Immunology (287)

Isbn Number

978-3-540-21494-6

Pages

199-227

Publisher

Springer (Heidelberg)

Brief description/objective

In this article, we describe the reverse genetic system that is based on the use of vaccinia virus cloning vectors. This system represents a generic approach to coronavirus reverse genetics and was first described for the generation of recombinant human coronavirus 229E representing a group I coronavirus. Subsequently, the same approach has been used to generate recombinant avian infectious bronchitis coronavirus and, recently, recombinant mouse hepatitis virus, representing group III and group II coronaviruses, respectively. We describe how vaccinia virus-mediated homologous recombination can be used to introduce specific mutations into the coronavirus genomic cDNA during its propagation in vaccinia virus and how recombinant coronaviruses can be isolated. Finally, we describe how the coronavirus reverse genetic system has now been extended to the generation of coronavirus replicon RNAs.