Prospective study of a panfungal PCR assay followed by sequencing, for the detection of fungal DNA in normally sterile specimens in a clinical setting: a complementary tool in the diagnosis of invasive fungal disease?

Publikation

Prospective study of a panfungal PCR assay followed by sequencing, for the detection of fungal DNA in normally sterile specimens in a clinical setting: a complementary tool in the diagnosis of invasive fungal disease?

Wissenschaftlicher Artikel/Review - 26.04.2013

Babouee Flury Baharak, Frei R, Battegay M, Savic Prince S, Bubendorf L, Sadowski-Cron C, Lardinois D, Elzi L, Goldenberger D, Weisser M

Zitation

Babouee Flury B, Frei R, Battegay M, Savic Prince S, Bubendorf L, Sadowski-Cron C, Lardinois D, Elzi L, Goldenberger D, Weisser M. Prospective study of a panfungal PCR assay followed by sequencing, for the detection of fungal DNA in normally sterile specimens in a clinical setting: a complementary tool in the diagnosis of invasive fungal disease?. Clin Microbiol Infect 2013; 19:E354-7.

Art

Wissenschaftlicher Artikel/Review (Englisch)

Zeitschrift

Clin Microbiol Infect 2013; 19

Veröffentlichungsdatum

26.04.2013

eISSN (Online)

1469-0691

Seiten

E354-7

Kurzbeschreibung/Zielsetzung

We prospectively analyzed 34 clinical biopsy samples from 23 patients with a suspected invasive fungal infection by fungal culture, histology and a panfungal PCR followed by sequencing. Results were compared to the composite diagnosis according the European Organization for Research and Treatment of Cancer (EORTC) criteria. In 34 samples, culture, histology and panfungal PCR were positive in 35%, 38% and 62%, respectively. On the sample level the panfungal PCR revealed a sensitivity of 69% and a specificity of 62.5% compared to proven IFI according postoperative EORTC criteria. On patient level, the sensitivity of the PCR approach was 100%, specificity 62.5%.