Prospective study of a panfungal PCR assay followed by sequencing, for the detection of fungal DNA in normally sterile specimens in a clinical setting: a complementary tool in the diagnosis of invasive fungal disease?

Publication

Prospective study of a panfungal PCR assay followed by sequencing, for the detection of fungal DNA in normally sterile specimens in a clinical setting: a complementary tool in the diagnosis of invasive fungal disease?

Journal Paper/Review - Apr 26, 2013

Babouee Flury Baharak, Frei R, Battegay M, Savic Prince S, Bubendorf L, Sadowski-Cron C, Lardinois D, Elzi L, Goldenberger D, Weisser M

Citation

Babouee Flury B, Frei R, Battegay M, Savic Prince S, Bubendorf L, Sadowski-Cron C, Lardinois D, Elzi L, Goldenberger D, Weisser M. Prospective study of a panfungal PCR assay followed by sequencing, for the detection of fungal DNA in normally sterile specimens in a clinical setting: a complementary tool in the diagnosis of invasive fungal disease?. Clin Microbiol Infect 2013; 19:E354-7.

Type

Journal Paper/Review (English)

Journal

Clin Microbiol Infect 2013; 19

Publication Date

Apr 26, 2013

Issn Electronic

1469-0691

Pages

E354-7

Brief description/objective

We prospectively analyzed 34 clinical biopsy samples from 23 patients with a suspected invasive fungal infection by fungal culture, histology and a panfungal PCR followed by sequencing. Results were compared to the composite diagnosis according the European Organization for Research and Treatment of Cancer (EORTC) criteria. In 34 samples, culture, histology and panfungal PCR were positive in 35%, 38% and 62%, respectively. On the sample level the panfungal PCR revealed a sensitivity of 69% and a specificity of 62.5% compared to proven IFI according postoperative EORTC criteria. On patient level, the sensitivity of the PCR approach was 100%, specificity 62.5%.