Publikation

Oncogenic hijacking of a developmental transcription factor evokes vulnerability toward oxidative stress in Ewing sarcoma

Wissenschaftlicher Artikel/Review - 15.05.2020

Bereiche
PubMed
DOI

Zitation
Marchetto A, Dallmayer M, Romero-Pérez L, Hölting T, Amatruda J, Cossarizza A, Henssen A, Kirchner T, Moretti M, Cidre-Aranaz F, Sannino G, Musa J, Baldauf M, Gerke J, Ohmura S, Orth M, Knott M, Colombo M, Arrigoni C, Bardinet V, Saucier D, Wehweck F, Li J, Stein S, Grünewald T. Oncogenic hijacking of a developmental transcription factor evokes vulnerability toward oxidative stress in Ewing sarcoma. Nat Commun 2020; 11:2423.
Art
Wissenschaftlicher Artikel/Review (Englisch)
Zeitschrift
Nat Commun 2020; 11
Veröffentlichungsdatum
15.05.2020
eISSN (Online)
2041-1723
Seiten
2423
Kurzbeschreibung/Zielsetzung

Ewing sarcoma (EwS) is an aggressive childhood cancer likely originating from mesenchymal stem cells or osteo-chondrogenic progenitors. It is characterized by fusion oncoproteins involving EWSR1 and variable members of the ETS-family of transcription factors (in 85% FLI1). EWSR1-FLI1 can induce target genes by using GGAA-microsatellites as enhancers.Here, we show that EWSR1-FLI1 hijacks the developmental transcription factor SOX6 - a physiological driver of proliferation of osteo-chondrogenic progenitors - by binding to an intronic GGAA-microsatellite, which promotes EwS growth in vitro and in vivo. Through integration of transcriptome-profiling, published drug-screening data, and functional in vitro and in vivo experiments including 3D and PDX models, we discover that constitutively high SOX6 expression promotes elevated levels of oxidative stress that create a therapeutic vulnerability toward the oxidative stress-inducing drug Elesclomol.Collectively, our results exemplify how aberrant activation of a developmental transcription factor by a dominant oncogene can promote malignancy, but provide opportunities for targeted therapy.