A quantitative immunohistochemical study on the time-dependent course of acute inflammatory cellular response to human brain injury

Publikation

A quantitative immunohistochemical study on the time-dependent course of acute inflammatory cellular response to human brain injury

Wissenschaftlicher Artikel/Review - 01.01.1999

Hausmann Roland, Kaiser A, Lang C, Bohnert M, Betz P

Bereiche

Rechtsmedizin

PubMed

10433032

Zitation

Hausmann R, Kaiser A, Lang C, Bohnert M, Betz P. A quantitative immunohistochemical study on the time-dependent course of acute inflammatory cellular response to human brain injury. Int J Legal Med 1999; 112:227-32.

Art

Wissenschaftlicher Artikel/Review (Englisch)

Zeitschrift

Int J Legal Med 1999; 112

Veröffentlichungsdatum

01.01.1999

ISSN (Druck)

0937-9827

Seiten

227-32

Kurzbeschreibung/Zielsetzung

The time-dependent inflammatory cell reaction in human cortical contusions has been investigated during the first 30 weeks after blunt head injury. Immunohistochemical staining was carried out using CD 15 for granulocytes and LCA, CD 3 and UCHL-1 for mononuclear leucocytes. In order to provide reliable data for a forensic wound age estimation, the intensity of the cellular reaction was evaluated with a quantitative image analysis system. CD 15-labelled granulocytes were detectable earliest 10 min after brain injury, whereas significantly increased numbers of mononuclear leucocytes occurred in cortical contusions after a postinfliction interval of at least 1.1 days (LCA), 2 days (CD 3) or 3.7 days (UCHL-1), respectively.