A strategy for the generation of infectious RNAs and autonomously replicating RNAs based on the HCV 229E genome

Publikation

A strategy for the generation of infectious RNAs and autonomously replicating RNAs based on the HCV 229E genome

Konferenzpapier/Poster - 01.01.1998

Herold J, Thiel Volker, Siddell S G

Bereiche

Institut für Immunbiologie

PubMed

9782291

Zitation

Herold J, Thiel V, Siddell S (1998). A strategy for the generation of infectious RNAs and autonomously replicating RNAs based on the HCV 229E genome.

Art

Konferenzpapier/Poster (Englisch)

Name der Konferenz

- (-)

Titel der Konferenzberichte

Advances in experimental medicine and biology (440)

Veröffentlichungsdatum

01.01.1998

ISSN (Druck)

0065-2598

Seiten

265-8

Verlag

Springer (Heidelberg)

Kurzbeschreibung/Zielsetzung

A strategy to generate in vitro transcripts representing infectious RNAs and autonomously replicating RNAs based on the HCV 229E genome is presented. PCR-DNAs were ligated in vitro, resulting in 27 kbp and 22 kbp ligation products. These DNAs can now be transcribed in vitro and the RNAs tested for infectivity and their ability to replicate.