Publikation

A novel cryopreservation and biobanking strategy to study lymphoid tissue stromal cells in human disease.

Wissenschaftlicher Artikel/Review - 27.06.2023

Bereiche
PubMed
DOI
Kontakt

Zitation
Brandstadter J, De Martin A, Lϋtge M, Ferreira A, Gaudette B, Stanossek Y, Wang S, Gonzalez M, Camiolo E, Wertheim G, Austin B, Allman D, Bagg A, Salim M, Fajgenbaum D, Aster J, Ludewig B, Maillard I. A novel cryopreservation and biobanking strategy to study lymphoid tissue stromal cells in human disease. Eur J Immunol 2023:e2250362.
Art
Wissenschaftlicher Artikel/Review (Englisch)
Zeitschrift
Eur J Immunol 2023
Veröffentlichungsdatum
27.06.2023
eISSN (Online)
1521-4141
Seiten
e2250362
Kurzbeschreibung/Zielsetzung

Nonhematopoietic lymph node stromal cells (LNSCs) regulate lymphocyte trafficking, survival, and function for key roles in host defense, autoimmunity, alloimmunity, and lymphoproliferative disorders. However, the study of LNSCs in human diseases is complicated by a dependence on viable lymphoid tissues, which are most often excised prior to establishment of a specific diagnosis. Here, we demonstrate that cryopreservation can be used to bank lymphoid tissue for the study of LNSCs in human disease. Using human tonsils and lymph nodes (LN), lymphoid tissue fragments were cryopreserved for subsequent enzymatic digestion and recovery of viable nonhematopoietic cells. Flow cytometry and single-cell transcriptomics identified comparable proportions of LN stromal cell types in fresh and cryopreserved tissue. Moreover, cryopreservation had little effect on transcriptional profiles, which showed significant overlap between tonsils and LN. The presence and spatial distribution of transcriptionally defined cell types were confirmed by in situ analyses. Our broadly applicable approach promises to greatly enable research into the roles of LNSCs in human disease.