Publikation

Protein fibril length in cerebrospinal fluid is increased in Alzheimer's disease.

Wissenschaftlicher Artikel/Review - 08.03.2023

Bereiche
PubMed
DOI
Kontakt

Zitation
Nirmalraj P, Schneider T, Lüder L, Felbecker A. Protein fibril length in cerebrospinal fluid is increased in Alzheimer's disease. Commun Biol 2023; 6:251.
Art
Wissenschaftlicher Artikel/Review (Englisch)
Zeitschrift
Commun Biol 2023; 6
Veröffentlichungsdatum
08.03.2023
eISSN (Online)
2399-3642
Seiten
251
Kurzbeschreibung/Zielsetzung

Alzheimer's disease (AD) associated proteins exist in cerebrospinal fluid (CSF). This paper evidences that protein aggregate morphology distinctly differs in CSF of patients with AD dementia (ADD), mild cognitive impairment due to AD (MCI AD), with subjective cognitive decline without amyloid pathology (SCD) and with non-AD MCI using liquid-based atomic force microscopy (AFM). Spherical-shaped particles and nodular-shaped protofibrils were present in the CSF of SCD patients, whereas CSF of ADD patients abundantly contained elongated mature fibrils. Quantitative analysis of AFM topographs confirms fibril length is higher in CSF of ADD than in MCI AD and lowest in SCD and non-AD dementia patients. CSF fibril length is inversely correlated with CSF amyloid beta (Aβ) 42/40 ratio and CSF p-tau protein levels (obtained from biochemical assays) to predict amyloid and tau pathology with an accuracy of 94% and 82%, respectively, thus identifying ultralong protein fibrils in CSF as a possible signature of AD pathology.