Automatic quantification of angiogenesis in 2D sections: a precise and timesaving approach.

Publikation

Automatic quantification of angiogenesis in 2D sections: a precise and timesaving approach.

Wissenschaftlicher Artikel/Review - 16.04.2015

Weisshaupt Christian, Covi J M, Hilgert J G, Leibig Nico, Arkudas Andreas, Horch R E, Kneser Ulrich, Schmidt Volker

Zitation

Weisshaupt C, Covi J, Hilgert J, Leibig N, Arkudas A, Horch R, Kneser U, Schmidt V. Automatic quantification of angiogenesis in 2D sections: a precise and timesaving approach. J Microsc 2015; 259:185-96.

Art

Wissenschaftlicher Artikel/Review (Englisch)

Zeitschrift

J Microsc 2015; 259

Veröffentlichungsdatum

16.04.2015

eISSN (Online)

1365-2818

Seiten

185-96

Kurzbeschreibung/Zielsetzung

The standardized characterization of angiogenesis is crucial in the field of tissue engineering as sufficient blood supply is the limiting factor of mass transfer. However, reliable algorithms that provide a straight forward and observer-independent assessment of new vessel formation are still lacking. We propose an automatic observer-independent quantitative method (including downloadable source code) to analyze vascularization using two-dimensional microscopic images of histological cross-sections and advanced postprocessing, based on a 'positive- and negative-experts' model and a (corrected) nearest neighbour classification, in a vascularized tissue engineering model.