Critical role for DNA vaccination frequency in induction of antigen-specific cytotoxic responses

Publikation

Critical role for DNA vaccination frequency in induction of antigen-specific cytotoxic responses

Wissenschaftlicher Artikel/Review - 27.02.2006

Heinzerling Lucie, Basch Veronique, Maloy Kevin, Johansen Pål, Senti Gabriela, Wüthrich Brunello, Storni Tazio, Kündig Thomas M

Zitation

Heinzerling L, Basch V, Maloy K, Johansen P, Senti G, Wüthrich B, Storni T, Kündig T. Critical role for DNA vaccination frequency in induction of antigen-specific cytotoxic responses. Vaccine 2006; 24:1389-94.

Art

Wissenschaftlicher Artikel/Review (Englisch)

Zeitschrift

Vaccine 2006; 24

Veröffentlichungsdatum

27.02.2006

ISSN (Druck)

0264-410X

Seiten

1389-94

Kurzbeschreibung/Zielsetzung

Since antigen-persistence plays a role for induction of immunity, we investigated the in vivo pharmacokinetic of a naked DNA vaccine at the site of its action, i.e., in the lymph node. After direct intralymphatic injection, naked DNA vaccine degraded within a few hours. In correlation with the short persistence of the DNA vaccine we found that the frequency of vaccination critically influenced the strength of the immune response. In mice vaccinated every 3 days, cytotoxic T-cell responses were enhanced compared to immunization in 6 or 9 days intervals. The results suggest that the so far disappointing efficiency of naked DNA vaccines in humans may be overcome by more frequent vaccination.