Array-based diagnostic gene-expression score for atopy and asthma

Publikation

Array-based diagnostic gene-expression score for atopy and asthma

Wissenschaftlicher Artikel/Review - 01.02.2002

Brutsche Martin, Joos Ladina, Carlen Brutsche Ingrid E A, Bissinger Roland, Tamm Michael, Custovic Adnan, Woodcock Ashley

Bereiche

Pneumologie und Schlafmedizin

PubMed

11842296

Zitation

Brutsche M, Joos L, Carlen Brutsche I, Bissinger R, Tamm M, Custovic A, Woodcock A. Array-based diagnostic gene-expression score for atopy and asthma. The Journal of allergy and clinical immunology 2002; 109:271-3.

Art

Wissenschaftlicher Artikel/Review (Englisch)

Zeitschrift

The Journal of allergy and clinical immunology 2002; 109

Veröffentlichungsdatum

01.02.2002

ISSN (Druck)

0091-6749

Seiten

271-3

Kurzbeschreibung/Zielsetzung

Whether gene expression is useful in discriminating different atopic phenotypes is unclear. The aim of the study was to evaluate a gene-expression score for the diagnosis of atopy and asthma and to assess disease activity as a guide for therapeutic decisions. Purified mRNA from PBMCs of 18 atopic asthmatic subjects, 8 atopic nonasthmatic subjects, and 14 healthy control subjects was hybridized to cDNA membranes. A composite atopy gene expression (CAGE) score was determined by using 10 genes dysregulated in atopic individuals according to a specific algorithm. The CAGE score was better than total IgE in differentiating atopic from nonatopic subjects (sensitivity, 96%; specificity, 92%). Correlation between the CAGE score and total IgE (P <.001) was found, and there was a trend for correlation with asthma severity (P =.051). The CAGE score was able to quantify phenotype-specific alteration in gene expression of atopic individuals. The CAGE score might be used as a diagnostic tool or to monitor the effects and side effects of therapy.