Array-based diagnostic gene-expression score for atopy and asthma

Publication

Array-based diagnostic gene-expression score for atopy and asthma

Journal Paper/Review - Feb 1, 2002

Brutsche Martin, Joos Ladina, Carlen Brutsche Ingrid E A, Bissinger Roland, Tamm Michael, Custovic Adnan, Woodcock Ashley

Units

Pneumologie und Schlafmedizin

PubMed

11842296

Citation

Brutsche M, Joos L, Carlen Brutsche I, Bissinger R, Tamm M, Custovic A, Woodcock A. Array-based diagnostic gene-expression score for atopy and asthma. The Journal of allergy and clinical immunology 2002; 109:271-3.

Type

Journal Paper/Review (English)

Journal

The Journal of allergy and clinical immunology 2002; 109

Publication Date

Feb 1, 2002

Issn Print

0091-6749

Pages

271-3

Brief description/objective

Whether gene expression is useful in discriminating different atopic phenotypes is unclear. The aim of the study was to evaluate a gene-expression score for the diagnosis of atopy and asthma and to assess disease activity as a guide for therapeutic decisions. Purified mRNA from PBMCs of 18 atopic asthmatic subjects, 8 atopic nonasthmatic subjects, and 14 healthy control subjects was hybridized to cDNA membranes. A composite atopy gene expression (CAGE) score was determined by using 10 genes dysregulated in atopic individuals according to a specific algorithm. The CAGE score was better than total IgE in differentiating atopic from nonatopic subjects (sensitivity, 96%; specificity, 92%). Correlation between the CAGE score and total IgE (P <.001) was found, and there was a trend for correlation with asthma severity (P =.051). The CAGE score was able to quantify phenotype-specific alteration in gene expression of atopic individuals. The CAGE score might be used as a diagnostic tool or to monitor the effects and side effects of therapy.