Publikation

Phages bearing affinity peptides to severe acute respiratory syndromes-associated coronavirus differentiate this virus from other viruses

Wissenschaftlicher Artikel/Review - 09.05.2013

Bereiche
PubMed
DOI

Zitation
Wang C, Sun X, Suo S, Ren Y, Li X, Herrler G, Thiel V, Ren X. Phages bearing affinity peptides to severe acute respiratory syndromes-associated coronavirus differentiate this virus from other viruses. J Clin Virol 2013; 57:305-10.
Art
Wissenschaftlicher Artikel/Review (Englisch)
Zeitschrift
J Clin Virol 2013; 57
Veröffentlichungsdatum
09.05.2013
eISSN (Online)
1873-5967
Seiten
305-10
Kurzbeschreibung/Zielsetzung

BACKGROUND
Transmission of SARS-associated coronavirus (SARS-CoV) is now well controlled, nevertheless, it is important to develop effective methods to identify this virus from other pathogens.

OBJECTIVES
The purpose of this study was to identify potential ligands and develop a novel diagnostic test to SARS-CoV using phage display technology.

STUDY DESIGN
The SARS-CoV spike 1 (S1) protein containing the receptor binding region (RBD) was used as an immobilized target followed by incubation with a 12-mer phage display random peptide library. After four rounds of biopanning, 10 monoclonal phages with specific binding activity to the S1-RBD protein were obtained and subjected to binding and diagnostic assays.

RESULTS
DNA sequencing showed that two phage displayed peptides HHKTWHPPVMHL (phage-H) and SQWHPRSASYPM (phage-S) that were specific ligands to the S1 protein. Moreover, the selected phage-H and phage-S were capable of differentiating SARS-CoV from other coronaviruses in indirect enzyme-linked immunosorbent assays.

CONCLUSION
The peptides identified in this study are useful reagents for detection of SARS-CoV.