Publication
Phages bearing affinity peptides to severe acute respiratory syndromes-associated coronavirus differentiate this virus from other viruses
Journal Paper/Review - May 9, 2013
Wang Chao, Sun Xuejiao, Suo Siqingaowa, Ren Yudong, Li Xunliang, Herrler Georg, Thiel Volker, Ren Xiaofeng
Units
PubMed
Doi
Citation
Type
Journal
Publication Date
Issn Electronic
Pages
Brief description/objective
BACKGROUND
Transmission of SARS-associated coronavirus (SARS-CoV) is now well controlled, nevertheless, it is important to develop effective methods to identify this virus from other pathogens.
OBJECTIVES
The purpose of this study was to identify potential ligands and develop a novel diagnostic test to SARS-CoV using phage display technology.
STUDY DESIGN
The SARS-CoV spike 1 (S1) protein containing the receptor binding region (RBD) was used as an immobilized target followed by incubation with a 12-mer phage display random peptide library. After four rounds of biopanning, 10 monoclonal phages with specific binding activity to the S1-RBD protein were obtained and subjected to binding and diagnostic assays.
RESULTS
DNA sequencing showed that two phage displayed peptides HHKTWHPPVMHL (phage-H) and SQWHPRSASYPM (phage-S) that were specific ligands to the S1 protein. Moreover, the selected phage-H and phage-S were capable of differentiating SARS-CoV from other coronaviruses in indirect enzyme-linked immunosorbent assays.
CONCLUSION
The peptides identified in this study are useful reagents for detection of SARS-CoV.