Publikation
Interferon-β Modulates the Innate Immune Response against Glioblastoma Initiating Cells
Wissenschaftlicher Artikel/Review - 06.10.2015
Wolpert Fabian, Weller Michael, Westphal Manfred, Lamszus Katrin, Neidert Marian Christoph, Roth Patrick, Deenen René, Florea Ana-Maria, Reifenberger Guido, Happold Caroline, Eisele Günter
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Immunotherapy targeting glioblastoma initiating cells (GIC) is considered a promising strategy. However, GIC are prone to evade immune response and there is a need for potent adjuvants. IFN-β might enhance the immune response and here we define its net effect on the innate immunogenicity of GIC. The transcriptomes of GIC treated with IFN-β and controls were assessed by microarray-based expression profiling for altered expression of immune regulatory genes. Several genes involved in adaptive and innate immune responses were regulated by IFN-β. We validated these results using reverse transcription (RT)-PCR and flow cytometry for corresponding protein levels. The up-regulation of the NK cell inhibitory molecules HLA-E and MHC class I was balanced by immune stimulating effects including the up-regulation of nectin-2. In 3 out of 5 GIC lines tested we found a net immune stimulating effect of IFN-β in cytotoxicity assays using NKL cells as effectors. IFN-β therefore warrants further investigation as an adjuvant for immunotherapy targeting GIC.