Projekt

Does hyper-thermal incubation and blockage of T-cell inhibitory pathways improve current and future T-cell based diagnostic test systems?

Abgeschlossen · 2011 bis 2014

Art
Grundlagenforschung
Reichweite
Monozentrisch am KSSG
Bereiche
Status
Abgeschlossen
Start
2011
Ende
2014
Finanzierungsart
Eigenfinanzierung
Schlagwörter (Tags)
CMI, LTBI, IGRA
Projektpartner
Dr. Mortren Ruhwald, TB Immunology Group, Hvidovre Hospital, Denmark TBNET
Kurzbeschreibung/Zielsetzung

Background: Cell mediated immune (CMI) assays are important research tools to monitor T-cell dependent immune responses towards specific antigens. Recently CMI assays were introduced for the diagnosis of latent tuberculosis infection (LTBI). In these assays interferon-gamma or alternatively interferon-gamma induced protein 10 (IP-10) production is quantified after M.tuberculosis-specific T-cell stimulation (Interferon-gamma Release Assys, IGRA). ÎGRA's are adapted to other clinical questions, e.g. detection of cytomegalovirus specific CMI in solid transplant recipients.

Hypothesis: It remains a dogma that IGRA are incubated at 37°C. Nevertheless fever is part of the innate immune response promoting antigen-presentation and pro-inflammatory cytokine release. The aim of the study is to explore IGRA test sensitivity / performance incubated at 39°C versus 37°C in different infectious diseases, in particular tuberculosis.