Publikation

Protective effect of focal adhesion kinase against skeletal muscle reperfusion injury after acute limb ischemia

Wissenschaftlicher Artikel/Review - 30.12.2014

Bereiche
PubMed
DOI

Zitation
Flück M, von Allmen R, Ferrié C, Tevaearai H, Dick F. Protective effect of focal adhesion kinase against skeletal muscle reperfusion injury after acute limb ischemia. Eur J Vasc Endovasc Surg 2014; 49:306-13.
Art
Wissenschaftlicher Artikel/Review (Englisch)
Zeitschrift
Eur J Vasc Endovasc Surg 2014; 49
Veröffentlichungsdatum
30.12.2014
eISSN (Online)
1532-2165
Seiten
306-13
Kurzbeschreibung/Zielsetzung

OBJECTIVES
In cardiac muscle, ischemia reperfusion (IR) injury is attenuated by mitochondrial function, which may be upregulated by focal adhesion kinase (FAK). The aim of this study was to determine whether increased FAK levels reduced rhabdomyolysis in skeletal muscle too.

MATERIAL AND METHODS
In a translational in vivo experiment, rat lower limbs were subjected to 4 hours of ischemia followed by 24 or 72 hours of reperfusion. FAK expression was stimulated 7 days before (via somatic transfection with pCMV-driven FAK expression plasmid) and outcomes were measured against non-transfected and empty transfected controls. Slow oxidative (i.e., mitochondria-rich) and fast glycolytic (i.e., mitochondria-poor) type muscles were analyzed separately regarding rhabdomyolysis, apoptosis, and inflammation. Severity of IR injury was assessed using paired non-ischemic controls.

RESULTS
After 24 hours of reperfusion, marked rhabdomyolysis was found in non-transfected and empty plasmid-transfected fast-type glycolytic muscle, tibialis anterior. Prior transfection enhanced FAK concentration significantly (p = 0.01). Concomitantly, levels of BAX, promoting mitochondrial transition pores, were reduced sixfold (p = 0.02) together with a blunted inflammation (p = 0.01) and reduced rhabdomyolysis (p = 0.003). Slow oxidative muscle, m. soleus, reacted differently: although apoptosis was detectable after IR, rhabdomyolysis did not appear before 72 hours of reperfusion; and FAK levels were not enhanced in ischemic muscle despite transfection (p = 0.66).

CONCLUSIONS
IR-induced skeletal muscle rhabdomyolysis is a fiber type-specific phenomenon that appears to be modulated by mitochondria reserves. Stimulation of FAK may exploit these reserves constituting a potential therapeutic approach to reduce tissue loss following acute limb IR in fast-type muscle.