Publikation

Reverse genetics of SARS-related coronavirus using vaccinia virus-based recombination

Wissenschaftlicher Artikel/Review - 07.03.2012

Bereiche
PubMed
DOI

Zitation
van den Worm S, Thiel V, Snijder E, Siddell S, Chang G, Dijkman R, Kuri T, Züst R, Weber F, Zevenhoven J, Eriksson K, Davidson A. Reverse genetics of SARS-related coronavirus using vaccinia virus-based recombination. PloS one 2012; 7:e32857.
Art
Wissenschaftlicher Artikel/Review (Englisch)
Zeitschrift
PloS one 2012; 7
Veröffentlichungsdatum
07.03.2012
eISSN (Online)
1932-6203
Seiten
e32857
Kurzbeschreibung/Zielsetzung

Severe acute respiratory syndrome (SARS) is a zoonotic disease caused by SARS-related coronavirus (SARS-CoV) that emerged in 2002 to become a global health concern. Although the original outbreak was controlled by classical public health measures, there is a real risk that another SARS-CoV could re-emerge from its natural reservoir, either in its original form or as a more virulent or pathogenic strain; in which case, the virus would be difficult to control in the absence of any effective antiviral drugs or vaccines. Using the well-studied SARS-CoV isolate HKU-39849, we developed a vaccinia virus-based SARS-CoV reverse genetic system that is both robust and biosafe. The SARS-CoV genome was cloned in separate vaccinia virus vectors, (vSARS-CoV-5prime and vSARS-CoV-3prime) as two cDNAs that were subsequently ligated to create a genome-length SARS-CoV cDNA template for in vitro transcription of SARS-CoV infectious RNA transcripts. Transfection of the RNA transcripts into permissive cells led to the recovery of infectious virus (recSARS-CoV). Characterization of the plaques produced by recSARS-CoV showed that they were similar in size to the parental SARS-CoV isolate HKU-39849 but smaller than the SARS-CoV isolate Frankfurt-1. Comparative analysis of replication kinetics showed that the kinetics of recSARS-CoV replication are similar to those of SARS-CoV Frankfurt-1, although the titers of virus released into the culture supernatant are approximately 10-fold less. The reverse genetic system was finally used to generate a recSARS-CoV reporter virus expressing Renilla luciferase in order to facilitate the analysis of SARS-CoV gene expression in human dendritic cells (hDCs). In parallel, a Renilla luciferase gene was also inserted into the genome of human coronavirus 229E (HCoV-229E). Using this approach, we demonstrate that, in contrast to HCoV-229E, SARS-CoV is not able to mediate efficient heterologous gene expression in hDCs.