Publikation

Early endosomal maturation of MHC class II molecules independently of cysteine proteases and H-2DM

Wissenschaftlicher Artikel/Review - 01.03.2000

Bereiche
PubMed
DOI

Zitation
Villadangos J, Driessen C, Shi G, Chapman H, Ploegh H. Early endosomal maturation of MHC class II molecules independently of cysteine proteases and H-2DM. The EMBO journal 2000; 19:882-91.
Art
Wissenschaftlicher Artikel/Review (Englisch)
Zeitschrift
The EMBO journal 2000; 19
Veröffentlichungsdatum
01.03.2000
ISSN (Druck)
0261-4189
Seiten
882-91
Kurzbeschreibung/Zielsetzung

Major histocompatibility complex (MHC) class II molecules bind and present to CD4(+) T cells peptides derived from endocytosed antigens. Class II molecules associate in the endoplasmic reticulum with invariant chain (Ii), which (i) mediates the delivery of the class II-Ii complexes into the endocytic compartments where the antigenic peptides are generated; and (ii) blocks the peptide-binding site of the class II molecules until they reach their destination. Once there, Ii must be removed to allow peptide binding. The bulk of Ii-class II complexes reach late endocytic compartments where Ii is eliminated in a reaction in which the cysteine protease cathepsin S and the accessory molecule H-2DM play an essential role. Here, we here show that Ii is also eliminated in early endosomal compartments without the intervention of cysteine proteases or H-2DM. The Ii-free class II molecules generated by this alternative mechanism first bind high molecular weight polypeptides and then mature into peptide-loaded complexes.