Publikation

Cell size of alveolar macrophages: an interspecies comparison

Wissenschaftlicher Artikel/Review - 01.09.1997

Bereiche
PubMed

Zitation
Krombach F, Münzing S, Allmeling A, Gerlach T, Behr J, Dörger M. Cell size of alveolar macrophages: an interspecies comparison. Environmental health perspectives 1997; 105 Suppl 5:1261-3.
Art
Wissenschaftlicher Artikel/Review (Englisch)
Zeitschrift
Environmental health perspectives 1997; 105 Suppl 5
Veröffentlichungsdatum
01.09.1997
ISSN (Druck)
0091-6765
Seiten
1261-3
Kurzbeschreibung/Zielsetzung

Alveolar macrophages (AM) play a critical role in the removal of inhaled particles or fibers from the lung. Species differences in AM size may affect the number and size range of particles/fibers that can be actually phagocytized and cleared by AM. The purpose of this study was to compare the cell size of rat, hamster, monkey, and human AM by selective flow cytometric analysis of cell volume. Resident AM from CD rats, Syrian golden hamsters, cynomolgus monkeys, and nonsmoking, healthy human volunteers were harvested by standard bronchoalveolar lavage procedures. Morphometric analysis of AM was performed using a flow cytometer that generates volume signals based on the Coulter-type measurement of electrical resistance. We found that hamster and rat AM had diameters of 13.6 +/- 0.4 microns (n = 8) and 13.1 +/- 0.2 microns (n = 12), respectively. Comparatively, the AM from monkeys (15.3 +/- 0.5 microns, n = 7) and human volunteers (21.2 +/- 0.3 microns, n = 10) were larger than those from rats and hamsters. The AM from humans were significantly larger (p < 0.05) than those from all other species studied, corresponding to a 4-fold larger cell volume of human AM (4990 +/- 174 microns 3) compared to hamster (1328 +/- 123 microns 3) and rat (1166 +/- 42 microns 3) AM. In summary, we have found marked species differences in the cell size of AM. We suggest that the number and size range of particles/fibers that can be phagocytized and cleared by AM may differ among species due to inherent or acquired species differences in AM cell size.