Publikation

Titanium uptake, induction of RANK-L expression, and enhanced proliferation of human T-lymphocytes

Wissenschaftlicher Artikel/Review - 06.10.2009

Bereiche
PubMed
DOI

Zitation
Cadosch D, Sutanto M, Chan E, Mhawi A, Gautschi O, von Katterfeld B, Simmen H, Filgueira L. Titanium uptake, induction of RANK-L expression, and enhanced proliferation of human T-lymphocytes. Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2009
Art
Wissenschaftlicher Artikel/Review (Englisch)
Zeitschrift
Journal of orthopaedic research : official publication of the Orthopaedic Research Society 2009
Veröffentlichungsdatum
06.10.2009
eISSN (Online)
1554-527X
Kurzbeschreibung/Zielsetzung

There is increasing evidence that titanium ions are released from orthopedic implants by biocorrosion. The aim of this study was to investigate titanium uptake by human T-lymphocytes and its effects on phenotype and proliferation. Freshly isolated human nonadherent peripheral blood mononuclear cells (NA-PBMC), were exposed to TiCl(4) [Ti(IV)]. Bioavailability and distribution of Ti(IV) in T-lymphocytes was determined by energy-filtered electron microscopy (EFTEM). The effects of Ti(IV) challenge on nonactivated and PHA-activated cells were assessed by flow cytometric analysis of surface markers, RANK-L production, and proliferation assays. EFTEM colocalized Ti(IV) with phosphorus in the nucleus, ribosomes, cytoplasmic membranes, and the surface membrane of T-lymphocytes. Ti(IV) increased significantly the expression of CD69, CCR4, and RANK-L in a concentration-dependent manner. Titanium enters T-lymphocytes through a currently unknown mechanism and binds to phosphorus-rich cell structures. Titanium influences phenotype and function of T-lymphocytes, resulting in activation of a CD69+ and CCR4+ T-lymphocyte population and secretion of RANK-L. These results strongly suggest the involvement of titanium ions challenged T-lymphocytes in the complex pathophysiological mechanisms of aseptic loosening of orthopedic implants. (c) 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res.