Kinetics of CXCR4 and CCR5 up-regulation and human immunodeficiency virus expansion after antigenic stimulation of primary CD4(+) T lymphocytes

Publication

Kinetics of CXCR4 and CCR5 up-regulation and human immunodeficiency virus expansion after antigenic stimulation of primary CD4(+) T lymphocytes

Journal Paper/Review - Sep 1, 2000

Maier Reinhard, Bartolomé-Rodríguez M M, Moulon C, Weltzien H U, Meyerhans A

Units

Medizinisches Forschungszentrum, Clinical Trials Unit

PubMed

10961886

Citation

Maier R, Bartolomé-Rodríguez M, Moulon C, Weltzien H, Meyerhans A. Kinetics of CXCR4 and CCR5 up-regulation and human immunodeficiency virus expansion after antigenic stimulation of primary CD4(+) T lymphocytes. Blood 2000; 96:1853-6.

Type

Journal Paper/Review (English)

Journal

Blood 2000; 96

Publication Date

Sep 1, 2000

Issn Print

0006-4971

Pages

1853-6

Brief description/objective

The chemokine receptors CCR5 and CXCR4 are coreceptors for the human immunodeficiency virus (HIV) and determine the cell tropism of different HIV strains. Previous studies on their regulation were performed under conditions of unspecific T-lymphocyte stimulation and provided conflicting results. To mimic physiologic conditions, highly purified primary Staphylococcus enterotoxin B (SEB)-reactive CD4 T lymphocytes were stimulated in the presence of autologous antigen-presenting cells and the kinetics of CCR5 and CXCR4 surface expression and HIV replication were studied. Both chemokine receptors were transiently up-regulated with maximal expression at day 3 after stimulation. The stimulated T cells were equally susceptible to productive infection with R5-and X4-tropic virus strains. Thus, antigenic stimulation of T cells promotes efficient replication of both, T cell-tropic and macrophage-tropic HIV. (Blood. 2000;96:1853-1856)