In vivo radionuclide uptake quantification using a multi-pinhole SPECT system to predict renal function in small animals

Publication

In vivo radionuclide uptake quantification using a multi-pinhole SPECT system to predict renal function in small animals

Journal Paper/Review - Jul 11, 2006

Forrer Flavio, Valkema R, Bernard B, Schramm N U, Hoppin J W, Rolleman E, Krenning E P, de Jong M

Citation

Forrer F, Valkema R, Bernard B, Schramm N, Hoppin J, Rolleman E, Krenning E, de Jong M. In vivo radionuclide uptake quantification using a multi-pinhole SPECT system to predict renal function in small animals. Eur J Nucl Med Mol Imaging 2006; 33:1214-7.

Type

Journal Paper/Review (English)

Journal

Eur J Nucl Med Mol Imaging 2006; 33

Publication Date

Jul 11, 2006

Issn Print

1619-7070

Pages

1214-7

Brief description/objective

PURPOSE
In vivo quantification of radiopharmaceuticals has great potential as a tool in developing new drugs. We investigated the accuracy of in vivo quantification with multi-pinhole single-photon emission computed tomography (SPECT) in rats.

METHODS
Fifteen male Lewis rats with different stages of renal dysfunction were injected with 50 MBq 99mTc-dimercaptosuccinic acid. Four to six hours after injection, SPECT of the kidneys was acquired with a new four-headed multi-pinhole collimator camera. Immediately after imaging the rats were sacrificed and the kidneys were counted in a gamma-counter to determine the absorbed activity. SPECT data were reconstructed iteratively and regions of interest (ROIs) were drawn manually. The absolute activity in the ROIs was determined.

RESULTS
Uptake values ranging from 0.71% to 21.87% of the injected activity were measured. A very strong linear correlation was found between the determined activity in vivo and ex vivo (r2=0.946; slope m=1.059).

CONCLUSION
Quantification in vivo using this multi-pinhole SPECT system is highly accurate.