Publication

IL-4Rα-Expressing B Cells Are Required for CXCL13 Production by Fibroblastic Reticular Cells

Journal Paper/Review - May 21, 2019

Units
PubMed
Doi

Citation
Dubey L, Ludewig B, Luther S, Harris N. IL-4Rα-Expressing B Cells Are Required for CXCL13 Production by Fibroblastic Reticular Cells. Cell Rep 2019; 27:2442-2458.e5.
Type
Journal Paper/Review (English)
Journal
Cell Rep 2019; 27
Publication Date
May 21, 2019
Issn Electronic
2211-1247
Pages
2442-2458.e5
Brief description/objective

Adaptive type 2 immune responses against the intestinal helminth Heligmosomoides polygyrus (Hp) require the interaction of follicle-associated CXCR5 dendritic cells with naive T cells in the draining mesenteric lymph nodes (mLNs). However, the source of CXCL13 responsible for attracting CXCR5 dendritic cells has remained unclear. Using multiplex imaging combined with deep tissue analysis, we observed new CXCL13 fibroblastic reticular cells surrounding paracortical and cortical B cell follicles in the mLNs of infected mice. CXCL13 fibroblasts expressed markers of marginal reticular cells (MRCs), and their expansion required lymphotoxin (LT)-dependent interactions between IL-4Rα-expressing B cells and CCL19 fibroblasts. Infection-induced follicles did not necessarily contain follicular dendritic cells (FDCs), indicating that CXCL13 fibroblasts may instead drive their formation. These data reveal a role for lymphotoxin signaling to CCL19 fibroblasts in the development of CXCL13 MRC-like cells and adaptive type 2 immunity in response to helminth infection.