A reverse genetics approach to study feline infectious peritonitis

Publication

A reverse genetics approach to study feline infectious peritonitis

Journal Paper/Review - Apr 4, 2012

Tekes Gergely, Spies Danica, Bank-Wolf Barbara, Thiel Volker, Thiel Heinz-Jürgen

Citation

Tekes G, Spies D, Bank-Wolf B, Thiel V, Thiel H. A reverse genetics approach to study feline infectious peritonitis. J Virol 2012; 86:6994-8.

Type

Journal Paper/Review (English)

Journal

J Virol 2012; 86

Publication Date

Apr 4, 2012

Issn Electronic

1098-5514

Pages

6994-8

Brief description/objective

Feline infectious peritonitis (FIP) is a lethal immunopathological disease caused by feline coronaviruses (FCoVs). Here, we describe a reverse genetics approach to study FIP by assessing the pathogenicity of recombinant type I and type II and chimeric type I/type II FCoVs. All recombinant FCoVs established productive infection in cats, and recombinant type II FCoV (strain 79-1146) induced FIP. Virus sequence analyses from FIP-diseased cats revealed that the 3c gene stop codon of strain 79-1146 has changed to restore a full-length open reading frame (ORF).