A quantitative immunohistochemical study on the time-dependent course of acute inflammatory cellular response to human brain injury

Publication

A quantitative immunohistochemical study on the time-dependent course of acute inflammatory cellular response to human brain injury

Journal Paper/Review - Jan 1, 1999

Hausmann Roland, Kaiser A, Lang C, Bohnert M, Betz P

Units

Rechtsmedizin

PubMed

10433032

Citation

Hausmann R, Kaiser A, Lang C, Bohnert M, Betz P. A quantitative immunohistochemical study on the time-dependent course of acute inflammatory cellular response to human brain injury. Int J Legal Med 1999; 112:227-32.

Type

Journal Paper/Review (English)

Journal

Int J Legal Med 1999; 112

Publication Date

Jan 1, 1999

Issn Print

0937-9827

Pages

227-32

Brief description/objective

The time-dependent inflammatory cell reaction in human cortical contusions has been investigated during the first 30 weeks after blunt head injury. Immunohistochemical staining was carried out using CD 15 for granulocytes and LCA, CD 3 and UCHL-1 for mononuclear leucocytes. In order to provide reliable data for a forensic wound age estimation, the intensity of the cellular reaction was evaluated with a quantitative image analysis system. CD 15-labelled granulocytes were detectable earliest 10 min after brain injury, whereas significantly increased numbers of mononuclear leucocytes occurred in cortical contusions after a postinfliction interval of at least 1.1 days (LCA), 2 days (CD 3) or 3.7 days (UCHL-1), respectively.