Sequence specific binding of the transcription factor c-Ets1 to the human immunodeficiency virus type I long terminal repeat

Publication

Sequence specific binding of the transcription factor c-Ets1 to the human immunodeficiency virus type I long terminal repeat

Journal Paper/Review - Dec 30, 1993

Holzmeister J, Ludewig Burkhard, Pauli G, Simon D

Citation

Holzmeister J, Ludewig B, Pauli G, Simon D. Sequence specific binding of the transcription factor c-Ets1 to the human immunodeficiency virus type I long terminal repeat. Biochemical and biophysical research communications 1993; 197:1229-33.

Type

Journal Paper/Review (English)

Journal

Biochemical and biophysical research communications 1993; 197

Publication Date

Dec 30, 1993

Issn Print

0006-291X

Pages

1229-33

Brief description/objective

Human immunodeficiency virus type I (HIV-1) long terminal repeat (LTR) driven transcription is regulated by a variety of cellular transcription factors. Most work has focused on the two nuclear factor kappa B (NF-kB) elements indispensable for HIV-1 LTR enhancer function. We demonstrate here the specific binding of the transcription factor c-Ets1 to an U3 region of the HIV-1 LTR (nt -141 to -149) using electrophoretic mobility shift analysis with T-cell nuclear extract and in vitro translated protein. This previously not identified Ets binding site is highly conserved among different HIV-1 isolates and maps to an U3 region recently shown to be necessary for viral growth in vitro. The c-Ets proto-oncogene family of transcription factors has yet been associated with HTLV-I and HIV-2 transcription. Our present analysis suggests an important role of c-Ets proteins in HIV-1 transcription.