Publication
A strategy for the generation of infectious RNAs and autonomously replicating RNAs based on the HCV 229E genome
Conference Paper/Poster - Jan 1, 1998
Herold J, Thiel Volker, Siddell S G
Units
PubMed
Citation
Herold J, Thiel V, Siddell S (1998). A strategy for the generation of infectious RNAs and autonomously replicating RNAs based on the HCV 229E genome.
Type
Conference Paper/Poster (English)
Conference Name
- (-)
Publisher Proceedings
Advances in experimental medicine and biology (440)
Publication Date
Jan 1, 1998
Issn Print
0065-2598
Pages
265-8
Publisher
Springer (Heidelberg)
Brief description/objective
A strategy to generate in vitro transcripts representing infectious RNAs and autonomously replicating RNAs based on the HCV 229E genome is presented. PCR-DNAs were ligated in vitro, resulting in 27 kbp and 22 kbp ligation products. These DNAs can now be transcribed in vitro and the RNAs tested for infectivity and their ability to replicate.