Genome organization and reverse genetic analysis of a type I feline coronavirus

Publication

Genome organization and reverse genetic analysis of a type I feline coronavirus

Journal Paper/Review - Feb 1, 2008

Tekes Gergely, Hofmann-Lehmann Regina, Stallkamp Iris, Thiel Volker, Thiel Heinz-Jürgen

Citation

Tekes G, Hofmann-Lehmann R, Stallkamp I, Thiel V, Thiel H. Genome organization and reverse genetic analysis of a type I feline coronavirus. Journal of virology 2008; 82:1851-9.

Type

Journal Paper/Review (English)

Journal

Journal of virology 2008; 82

Publication Date

Feb 1, 2008

Issn Electronic

1098-5514

Pages

1851-9

Brief description/objective

In this study we report the complete sequence and genome organization of the serotype I feline coronavirus (FCoV) strain Black. Furthermore, a reverse genetic system was established for this FCoV strain by cloning a full-length cDNA copy into vaccinia virus. This clone served as basis for the generation of recombinant FCoV (recFCoV) that was shown to bear the same features in vitro as the parental FCoV. Using this system, accessory 3abc genes in the FCoV genome were replaced by green fluorescent protein (recFCoV-GFP) and Renilla luciferase genes (recFCoV-RL). In addition, we showed that feline CD14(+) blood monocytes and dendritic cells can be easily detected after infection with recFCoV-GFP. Thus, our established reverse genetic system provides a suitable tool to study the molecular biology of serotype I FCoV.