Automatic quantification of angiogenesis in 2D sections: a precise and timesaving approach.

Publication

Automatic quantification of angiogenesis in 2D sections: a precise and timesaving approach.

Journal Paper/Review - Apr 16, 2015

Weisshaupt Christian, Covi J M, Hilgert J G, Leibig Nico, Arkudas Andreas, Horch R E, Kneser Ulrich, Schmidt Volker

Citation

Weisshaupt C, Covi J, Hilgert J, Leibig N, Arkudas A, Horch R, Kneser U, Schmidt V. Automatic quantification of angiogenesis in 2D sections: a precise and timesaving approach. J Microsc 2015; 259:185-96.

Type

Journal Paper/Review (English)

Journal

J Microsc 2015; 259

Publication Date

Apr 16, 2015

Issn Electronic

1365-2818

Pages

185-96

Brief description/objective

The standardized characterization of angiogenesis is crucial in the field of tissue engineering as sufficient blood supply is the limiting factor of mass transfer. However, reliable algorithms that provide a straight forward and observer-independent assessment of new vessel formation are still lacking. We propose an automatic observer-independent quantitative method (including downloadable source code) to analyze vascularization using two-dimensional microscopic images of histological cross-sections and advanced postprocessing, based on a 'positive- and negative-experts' model and a (corrected) nearest neighbour classification, in a vascularized tissue engineering model.