Publication

Aurora kinases A and B are up-regulated by Myc and are essential for maintenance of the malignant state

Journal Paper/Review - Jun 2, 2010

Units
PubMed
Doi

Citation
den Hollander J, Nilsson J, Cleveland J, Peschel C, Duyster J, von Bubnoff N, Goga A, Hall M, Scheerer M, Graf N, Kremer M, Hoellein A, Buck A, Rudelius M, Doherty J, Rimpi S, Keller U. Aurora kinases A and B are up-regulated by Myc and are essential for maintenance of the malignant state. Blood 2010; 116:1498-505.
Type
Journal Paper/Review (English)
Journal
Blood 2010; 116
Publication Date
Jun 2, 2010
Issn Electronic
1528-0020
Pages
1498-505
Brief description/objective

Myc oncoproteins promote continuous cell growth, in part by controlling the transcription of key cell cycle regulators. Here, we report that c-Myc regulates the expression of Aurora A and B kinases (Aurka and Aurkb), and that Aurka and Aurkb transcripts and protein levels are highly elevated in Myc-driven B-cell lymphomas in both mice and humans. The induction of Aurka by Myc is transcriptional and is directly mediated via E-boxes, whereas Aurkb is regulated indirectly. Blocking Aurka/b kinase activity with a selective Aurora kinase inhibitor triggers transient mitotic arrest, polyploidization, and apoptosis of Myc-induced lymphomas. These phenotypes are selectively bypassed by a kinase inhibitor-resistant Aurkb mutant, demonstrating that Aurkb is the primary therapeutic target in the context of Myc. Importantly, apoptosis provoked by Aurk inhibition was p53 independent, suggesting that Aurka/Aurkb inhibitors will show efficacy in treating primary or relapsed malignancies having Myc involvement and/or loss of p53 function.