[Round robin test for detection of genomic aberrations in non-Hodgkin lymphoma by in situ hybridization]

Publication

[Round robin test for detection of genomic aberrations in non-Hodgkin lymphoma by in situ hybridization]

Journal Paper/Review - Jul 1, 2013

Barth T F E, Thorns C, Stein H, Rosenwald A, Möller P, Mattfeldt T, Kradolfer D, Klapper W, Horn H, Hartmann S, Hansmann M L, Feller A C, Cogliatti Sergio B., Buck M, Bob R, Bernd H-W, Floßbach L, Ott G

Citation

Barth T, Thorns C, Stein H, Rosenwald A, Möller P, Mattfeldt T, Kradolfer D, Klapper W, Horn H, Hartmann S, Hansmann M, Feller A, Cogliatti S, Buck M, Bob R, Bernd H, Floßbach L, Ott G. [Round robin test for detection of genomic aberrations in non-Hodgkin lymphoma by in situ hybridization]. Pathologe 2013; 34:329-34.

Type

Journal Paper/Review (Deutsch)

Journal

Pathologe 2013; 34

Publication Date

Jul 1, 2013

Issn Electronic

1432-1963

Pages

329-34

Brief description/objective

BACKGROUND
The detection of characteristic genomic aberrations by fluorescence in situ hybridization (FISH) has a high diagnostic impact on lymphomas according to the World Health Organization (WHO). To investigate the reproducibility of non-isotopic ISH results a multicenter trial was carried out involving eight institutes for hematopathology.

MATERIAL AND METHODS
Analyses were performed on two diffuse large B-cell lymphomas (DLBCL) without known aberrations, on one follicular lymphoma with a IGH/BCL2 translocation and BCL6 split and on two B-cell lymphomas intermediate between DLBCL and Burkitt's lymphoma with c-MYC and BCL2 rearrangements, one with an additional BCL6 split. Break-apart probes for BCL6 and c-MYC, as well as fusion probes for the c-MYC/IGH and the IGH/BCL2 translocations were used.

RESULTS
All aberrations were correctly detected by all centres and no false positive or false negative results were obtained. The numbers of positive cells varied from 25% to 94%. Pearson's correlation coefficient between the centres was always > 0.8.

CONCLUSIONS
The ISH analysis of recurrent genomic aberrations in formalin-fixed paraffin-embedded (FFPE) tissue is a highly reproducible technique which yields substantial additive help for lymphoma diagnostics.