Human parechovirus type 1, 3, 4, 5, and 6 detection in picornavirus cultures

Publication

Human parechovirus type 1, 3, 4, 5, and 6 detection in picornavirus cultures

Journal Paper/Review - Dec 12, 2007

de Vries Michel, Pyrc Krzysztof, Berkhout Ron, Vermeulen-Oost Wilma, Dijkman Ronald, Jebbink Maarten F, Bruisten Sylvia, Berkhout Ben, van der Hoek Lia

Citation

de Vries M, Pyrc K, Berkhout R, Vermeulen-Oost W, Dijkman R, Jebbink M, Bruisten S, Berkhout B, van der Hoek L. Human parechovirus type 1, 3, 4, 5, and 6 detection in picornavirus cultures. J Clin Microbiol 2007; 46:759-62.

Type

Journal Paper/Review (English)

Journal

J Clin Microbiol 2007; 46

Publication Date

Dec 12, 2007

Issn Electronic

1098-660X

Pages

759-62

Brief description/objective

Picornavirus cultures that could not be typed in neutralization assays were analyzed by VP1 reverse transcription-PCR (RT-PCR) and a virus discovery tool (VIDISCA). Human parechoviruses (HPeVs) were frequently identified, among which were the uncommon isolates HPeV-4, HPeV-5, and HPeV-6. The HPeV-5 isolate could be amplified only by VIDISCA and not by VP1 RT-PCR.