Monocyte-derived dendritic cells represent a transient stage of differentiation in the myeloid lineage

Publication

Monocyte-derived dendritic cells represent a transient stage of differentiation in the myeloid lineage

Journal Paper/Review - Nov 1, 1997

Häusser G, Ludewig Burkhard, Gelderblom H R, Tsunetsugu-Yokota Y, Akagawa K, Meyerhans A

Units

Medizinisches Forschungszentrum, Institute of Immunobiology

PubMed

9413752

Citation

Häusser G, Ludewig B, Gelderblom H, Tsunetsugu-Yokota Y, Akagawa K, Meyerhans A. Monocyte-derived dendritic cells represent a transient stage of differentiation in the myeloid lineage. Immunobiology 1997; 197:534-42.

Type

Journal Paper/Review (English)

Journal

Immunobiology 1997; 197

Publication Date

Nov 1, 1997

Issn Print

0171-2985

Pages

534-42

Brief description/objective

Cultivation of human peripheral blood monocytes with granulocyte/macrophage colony stimulating factor (GM-CSF) and IL-4 facilitates generation of strongly antigen-presenting dendritic cells (DC). These monocyte-derived DC (mdDC) were used here to further delineate differentiation pathways in the myeloid lineage. Incubation of mdDC with TNF or soluble CD40L led to enhanced MHC and accessory surface antigen expression with significantly elevated T cell stimulatory activity, indicative of DC maturation. In contrast, after cytokine withdrawal or incubation with M-CSF, mdDC differentiated to macrophages. Cells became adherent, monocyte/macrophage surface markers were upregulated, and MHC and accessory surface proteins were downregulated. Furthermore, the multilaminar MHC class II compartments (MIIC) were lost and the T cell stimulating capacity largely diminished. Thus, mdDC show a high developmental plasticity by retaining their ability to become macrophages or to continue their differentiation towards mature DC.