Cut-offs for relapse detection in men with stage I testicular germ cell tumors during active surveillance within a prospective multicentre cohort study using either raw or housekeeper normalized miR-371a-3p serum levels.

Publication

Cut-offs for relapse detection in men with stage I testicular germ cell tumors during active surveillance within a prospective multicentre cohort study using either raw or housekeeper normalized miR-371a-3p serum levels.

Journal Paper/Review - Sep 10, 2024

Fankhauser Christian Daniel, Wettstein Marian S, Christiansen Ailsa J, Rothermundt Christian, Cathomas Richard, Kaufmann Ernest, Sigg Silvan, Templeton Arnoud J, Hirschi-Blickenstorfer Anita, Lorch Anja, Gillessen Silke, Beyer Jörg, Hermanns Thomas

Citation

Fankhauser C, Wettstein M, Christiansen A, Rothermundt C, Cathomas R, Kaufmann E, Sigg S, Templeton A, Hirschi-Blickenstorfer A, Lorch A, Gillessen S, Beyer J, Hermanns T. Cut-offs for relapse detection in men with stage I testicular germ cell tumors during active surveillance within a prospective multicentre cohort study using either raw or housekeeper normalized miR-371a-3p serum levels. Urol Oncol 2024; 42:455.e9-455.e13.

Type

Journal Paper/Review (English)

Journal

Urol Oncol 2024; 42

Publication Date

Sep 10, 2024

Issn Electronic

1873-2496

Pages

455.e9-455.e13

Brief description/objective

MiR-371a-3p represents a novel liquid biomarker that detects all histologies of germ-cell tumors (GCT) except teratoma. However, it is currently unclear whether miR-371a-3p results obtained directly from RT-PCR (raw Cq) or normalized for housekeeper genes and transformed into the relative quantity (RQ) value should be used and at what cut-off level. The purpose of this research was to evaluate, which values should be used, and a potential cut-off level for relapse-detection to inform subsequent studies.