Publication

Ki-67 as a prognostic marker in mantle cell lymphoma-consensus guidelines of the pathology panel of the European MCL Network

Journal Paper/Review - Jun 16, 2009

Units
PubMed
Doi

Citation
Klapper W, Möller P, Barth T, Muller-Hermelink K, Rosenwald A, Ott G, Pileri S, Ralfkiaer E, Rymkiewicz G, van Krieken J, Wacker H, Unterhalt M, Hiddemann W, Dreyling M, Stein H, Loddenkemper C, Hoster E, Determann O, Oschlies I, van der Laak J, Berger F, Bernd H, Cabeçadas J, Campo E, Cogliatti S, Hansmann M, Kluin P, Kodet R, Krivolapov Y, for the European MCL Network. Ki-67 as a prognostic marker in mantle cell lymphoma-consensus guidelines of the pathology panel of the European MCL Network. Journal of hematopathology 2009
Type
Journal Paper/Review (English)
Journal
Journal of hematopathology 2009
Publication Date
Jun 16, 2009
Issn Print
1865-5785
Brief description/objective

Mantle cell lymphoma (MCL) has a heterogeneous clinical course and is mainly an aggressive B cell non-Hodgkin lymphoma; however, there are some indolent cases The Ki-67 index, defined by the percentage of Ki-67-positive lymphoma cells on histopathological slides, has been shown to be a very powerful prognostic biomarker. The pathology panel of the European MCL Network evaluated methods to assess the Ki-67 index including stringent counting, digital image analysis, and estimation by eyeballing. Counting of 2 x 500 lymphoma cells is the gold standard to assess the Ki-67 index since this value has been shown to predict survival in prospective randomized trials of the European MCL Network. Estimation by eyeballing and digital image analysis showed a poor concordance with the gold standard (concordance correlation coefficients [CCC] between 0.29 and 0.61 for eyeballing and CCC of 0.24 and 0.37 for two methods of digital image analysis, respectively). Counting a reduced number of lymphoma cells (2 x 100 cells) showed high interobserver agreement (CCC = 0.74). Pitfalls of the Ki-67 index are discussed and guidelines and recommendations for assessing the Ki-67 index in MCL are given.