Publication

The second most abundant dinophyte in the ponds of a botanical garden is a species new to science.

Journal Paper/Review - Dec 11, 2023

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PubMed
Doi
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Citation
Müller A, Stark M, Schottenhammel S, John U, Chacón J, Klingl A, Holzer V, Schöffer M, Gottschling M. The second most abundant dinophyte in the ponds of a botanical garden is a species new to science. J Eukaryot Microbiol 2023:e13015.
Type
Journal Paper/Review (English)
Journal
J Eukaryot Microbiol 2023
Publication Date
Dec 11, 2023
Issn Electronic
1550-7408
Pages
e13015
Brief description/objective

In the microscopy realm, a large body of dark biodiversity still awaits to be uncovered. Unarmoured dinophytes are particularly neglected here, as they only present inconspicuous traits. In a remote German locality, we collected cells, from which a monoclonal strain was established, to study morphology using light and electron microscopy and to gain DNA sequences from the rRNA operon. In parallel, we detected unicellular eukaryotes in ponds of the Botanical Garden Munich-Nymphenburg by DNA-metabarcoding (V4 region of the 18S rRNA gene), weekly sampled over the course of a year. Strain GeoK*077 turned out to be a new species of Borghiella with a distinct position in molecular phylogenetics and characteristic coccoid cells of ovoid shape as the most important diagnostic trait. Borghiella ovum, sp. nov., was also present in artificial ponds of the Botanical Garden and was the second most abundant dinophyte detected in the samples. More specifically, Borghiella ovum, sp. nov., shows a clear seasonality, with high frequency during winter months and complete absence during summer months. The study underlines the necessity to assess the biodiversity, particularly of the microscopy realm more ambitiously, if even common species such as formerly Borghiella ovum are yet unknown to science.