Publication
Advanced Lymph Node Staging with Ex Vivo Intraarterial Indigo Carmine Injection after Transanal Total Mesorectal Excision for Rectal Cancer: A Retrospective Cohort Study
Journal Paper/Review - Nov 29, 2021
Widmann Bernhard, Almarie Bassel, Warschkow Rene, Beutner Ulrich, Weitzendorfer Michael, Schmid Michael, Ukegjini Kristjan, Brunner Walter
Units
Keywords
Humans
*Indigo Carmine
Lymph Nodes/pathology
Neoplasm Staging
*Rectal Neoplasms/pathology/surgery
Retrospective Studies
PubMed
Doi
Contact
Citation
Type
Journal
Publication Date
Issn Electronic
Pages
Brief description/objective
BACKGROUND
Exact lymph node staging is essential in rectal cancer therapy.
OBJECTIVE
The aim of the study was to assess the impact of intraarterial indigo carmine injection after transanal total mesorectal excision on the number of retrieved lymph nodes.
DESIGN
Retrospective, non-randomized study.
SETTINGS
The study was conducted at a tertiary hospital by a multidisciplinary team.
PATIENTS
Patients who underwent transanal total mesorectal excision for suspected rectal cancer between 2013 and 2019.
INTERVENTIONS
Rectal cancer specimen received ex vivo intraarterial indigo carmine injection to stain lymph nodes.
MAIN OUTCOME MEASURES
The number of retrieved lymph nodes with or without staining.
RESULTS
Specimens of 189 patients were analyzed, of which 108 (57.1%) were stained with indigo carmine. A mean of 19.8 ± 6.1 lymph nodes was identified in stained samples compared to 16.0 ± 4.9 without staining (p < 0.001). Multivariable analysis showed that 3.2 additional lymph nodes were found in stained specimens (95% confidence interval: 1.0 to 5.3; p = 0.02). In stained specimens the adequate lymph node count (≥12) was increased in univariable (odds ratio: 3.24, 95% confidence interval: 1.13 to 10.65; p = 0.03) but not in multivariable analysis. Indigo carmine injection had no effect on the number of positive lymph nodes or the nodal stage. Chemoradiotherapy reduced the lymph node count by 2.5 (p = 0.008). After staining, 95.0% of patients with chemoradiotherapy had ≥12 lymph nodes retrieved. The median follow-up of patients was 24.2 months with a local recurrence rate of 3.3%.
LIMITATIONS
The study is limited by its retrospective design and the non-randomized allocation.
CONCLUSIONS
Ex vivo intra-arterial indigo carmine injection increases the number of isolated lymph nodes after transanal total mesorectal excision regardless of neoadjuvant chemoradiotherapy. Indigo carmine injection is not associated with nodal upstaging or increased number of tumor positive lymph nodes. See Video Abstract at http://links.lww.com/DCR/B839.