Publication

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Journal Paper/Review - Oct 1, 2019

Units
Keywords
PubMed
Doi
Link
Contact

Citation
Cossarizza A, Chang H, Radbruch A, Acs A, Adam D, Adam-Klages S, Agace W, Aghaeepour N, Akdis M, Allez M, Almeida L, Alvisi G, Anderson G, Andrä I, Annunziato F, Anselmo A, Bacher P, Baldari C, Bari S, Barnaba V, Barros-Martins J, Battistini L, Bauer W, Baumgart S, Baumgarth N, Baumjohann D, Baying B, Bebawy M, Becher B, Beisker W, Benes V, Beyaert R, Blanco A, Boardman D, Bogdan C, Borger J, Borsellino G, Boulais P, Bradford J, Brenner D, Brinkman R, Brooks A, Busch D, Büscher M, Bushnell T, Calzetti F, Cameron G, Cammarata I, Cao X, Cardell S, Casola S, Cassatella M, Cavani A, Celada A, Chatenoud L, Chattopadhyay P, Chow S, Christakou E, Cicin-Sain L, Clerici M, Colombo F, Cook L, Cooke A, Cooper A, Corbett A, Cosma A, Cosmi L, Coulie P, Cumano A, Cvetkovic L, Dang V, Dang-Heine C, Davey M, Davies D, De Biasi S, Del Zotto G, Dela Cruz G, Delacher M, Della Bella S, Dellabona P, Deniz G, Dessing M, Di Santo J, Diefenbach A, Dieli F, Dolf A, Dörner T, Dress R, Dudziak D, Dustin M, Dutertre C, Ebner F, Eckle S, Edinger M, Eede P, Ehrhardt G, Eich M, Engel P, Engelhardt B, Erdei A, Esser C, Everts B, Evrard M, Falk C, Fehniger T, Felipo-Benavent M, Ferry H, Feuerer M, Filby A, Filkor K, Fillatreau S, Follo M, Förster I, Foster J, Foulds G, Frehse B, Frenette P, Frischbutter S, Fritzsche W, Galbraith D, Gangaev A, Garbi N, Gaudilliere B, Gazzinelli R, Geginat J, Gerner W, Gherardin N, Ghoreschi K, Gibellini L, Ginhoux F, Goda K, Godfrey D, Goettlinger C, González-Navajas J, Goodyear C, Gori A, Grogan J, Grummitt D, Grützkau A, Haftmann C, Hahn J, Hammad H, Hämmerling G, Hansmann L, Hansson G, Harpur C, Hartmann S, Hauser A, Hauser A, Haviland D, Hedley D, Hernández D, Herrera G, Herrmann M, Hess C, Höfer T, Hoffmann P, Hogquist K, Holland T, Höllt T, Holmdahl R, Hombrink P, Houston J, Hoyer B, Huang B, Huang F, Huber J, Huehn J, Hundemer M, Hunter C, Hwang W, Iannone A, Ingelfinger F, Ivison S, Jäck H, Jani P, Jávega B, Jonjic S, Kaiser T, Kalina T, Kamradt T, Kaufmann S, Keller B, Ketelaars S, Khalilnezhad A, Khan S, Kisielow J, Klenerman P, Knopf J, Koay H, Kobow K, Kolls J, Kong W, Kopf M, Korn T, Kriegsmann K, Kristyanto H, Kroneis T, Krueger A, Kühne J, Kukat C, Kunkel D, Kunze-Schumacher H, Kurosaki T, Kurts C, Kvistborg P, Kwok I, Landry J, Lantz O, Lanuti P, LaRosa F, Lehuen A, LeibundGut-Landmann S, Leipold M, Leung L, Levings M, Lino A, Liotta F, Litwin V, Liu Y, Ljunggren H, Lohoff M, Lombardi G, Lopez L, López-Botet M, Lovett-Racke A, Lubberts E, Luche H, Ludewig B, Lugli E, Lunemann S, Maecker H, Maggi L, Maguire O, Mair F, Mair K, Mantovani A, Manz R, Marshall A, Martínez-Romero A, Martrus G, Marventano I, Maslinski W, Matarese G, Mattioli A, Maueröder C, Mazzoni A, McCluskey J, McGrath M, McGuire H, McInnes I, Mei H, Melchers F, Melzer S, Mielenz D, Miller S, Mills K, Minderman H, Mjösberg J, Moore J, Moran B, Moretta L, Mosmann T, Müller S, Multhoff G, Munoz L, Münz C, Nakayama T, Nasi M, Neumann K, Ng L, Niedobitek A, Nourshargh S, Núñez G, O'Connor J, Ochel A, Oja A, Ordonez D, Orfao A, Orlowski-Oliver E, Ouyang W, Oxenius A, Palankar R, Panse I, Pattanapanyasat K, Paulsen M, Pavlinic D, Penter L, Peterson P, Peth C, Petriz J, Piancone F, Pickl W, Piconese S, Pinti M, Pockley A, Podolska M, Poon Z, Pracht K, Prinz I, Pucillo C, Quataert S, Quatrini L, Quinn K, Radbruch H, Radstake T, Rahmig S, Rahn H, Rajwa B, Ravichandran G, Raz Y, Rebhahn J, Recktenwald D, Reimer D, Reis e Sousa C, Remmerswaal E, Richter L, Rico L, Riddell A, Rieger A, Robinson J, Romagnani C, Rubartelli A, Ruland J, Saalmüller A, Saeys Y, Saito T, Sakaguchi S, Sala-de-Oyanguren F, Samstag Y, Sanderson S, Sandrock I, Santoni A, Sanz R, Saresella M, Sautes-Fridman C, Sawitzki B, Schadt L, Scheffold A, Scherer H, Schiemann M, Schildberg F, Schimisky E, Schlitzer A, Schlosser J, Schmid S, Schmitt S, Schober K, Schraivogel D, Schuh W, Schüler T, Schulte R, Schulz A, Schulz S, Scotta C, Scott-Algara D, Sester D, Shankey T, Silva-Santos B, Galler S, Sitnik K, Sozzani S, Speiser D, Spidlen J, Stahlberg A, Stall A, Stanley N, Stark R, Stehle C, Steinmetz T, Stockinger H, Takahama Y, Takeda K, Tan L, Tarnok A, Tiegs G, Toldi G, Tornack J, Traggiai E, Trebak M, Tree T, Trotter J, Trowsdale J, Tsoumakidou M, Ulrich H, Urbanczyk S, van de Veen W, van den Broek M, van der Pol E, Van Gassen S, Van Isterdael G, van Lier R, Veldhoen M, Vento-Asturias S, Vieira P, Voehringer D, Volk H, von Borstel A, von Volkmann K, Waisman A, Walker R, Wallace P, Wang S, Wang X, Ward M, Ward-Hartstonge K, Warnatz K, Warnes G, Warth S, Waskow C, Watson J, Watzl C, Wegener L, Weisenburger T, Wiedemann A, Wienands J, Wilharm A, Wilkinson R, Willimsky G, Wing J, Winkelmann R, Winkler T, Wirz O, Wong A, Wurst P, Yang J, Yang J, Yazdanbakhsh M, Yu L, Yue A, Zhang H, Zhao Y, Ziegler S, Zielinski C, Zimmermann J, Zychlinsky A. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). Eur J Immunol 2019; 49:1457-1973.
Project
Type
Journal Paper/Review (English)
Journal
Eur J Immunol 2019; 49
Publication Date
Oct 1, 2019
Issn Print
Issn Electronic
1521-4141
Pages
1457-1973
Publisher
Brief description/objective

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.